Removal of UV photoproducts from an adenovirus-encoded reporter gene following infection of unirradiated and UV-irradiated human fibroblasts.
作者： Ihor P. Boszko , Andrew J. Rainbow
关键词:
摘要: Ad5HCMVsp1lacZ is a recombinant nonreplicating adenovirus containing the lacZ gene under control of human cytomegalovirus immediate early promoter. Previous reports show that expression for UV-irradiated greater in nucleotide excision repair (NER) proficient compared to NER deficient fibroblasts from patients with xeroderma pigmentosum (XP) and Cockaye's syndrome (CS) pre-UV-treatment normal results an enhanced Ad5HCMVsp1lacZ. We have used quantitative PCR technique examine whether UV photoproducts are actually removed following infection Primers flanking 2.6-kb region reporter were added equal amounts DNA extracted infected cells amplified by using radiolabelled nucleotides as substrates. Results significant removal fibroblasts, but reduced XP CS pre-UV-treated fibroblasts.
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