A human/mouse chimeric monoclonal antibody against CA125 for radioimmunoimaging of ovarian cancer
作者： Kaichiro Ishibashi , Takeshi Watanabe , Keigo Endo , Isamu Ishiwata , Junji Konishi
DOI: 10.1007/BF01525427
关键词:
摘要: Murine monoclonal antibody 196-14 recognizes the ovarian-cancer-associated antigen CA 125, but epitope it is different from that of OC125. We developed a human/mouse chimeric using variable regions murine and human heavy-chain (γl) light-chain (κ) constant regions. Cell binding competitive inhibition assays labeled with125I,111In or99mTc demonstrated in vitro immunoreactivity was identical to parental antibody. However, mice bearing ovarian cancer xenografts, clearance blood faster absolute levels accumulation tumor were lower for the125I-labeled or99mTc-labeled than with corresponding radionuclides. The tumor-to-blood radioactivity ratio not significantly between antibody, regardless radionuclide used labeling. Chimeric with131I,111In promising radioimmunoimaging cancer.
springer.com
sci-hub.se 参考文章(30)
D V Morrissey, B A Stone, C A Guindon, H Dorai, G L Davis, J Saltzgaber-Muller, F Toneguzzo, S P Lilly, K W McDonald, B G Sahagan, A genetically engineered murine/human chimeric antibody retains specificity for human tumor-associated antigen. Journal of Immunology. ,vol. 137, pp. 1066- 1074 ,(1986)
Wilma M. McKoy, Karen R. Grotzinger, Elizabeth W. Chu, William R. Green, Robert C. Young, Jacqueline Whang-Peng, Alfred M. Rogan, John A. Green, Robert F. Ozols, Thomas C. Hamilton, Characterization of a Human Ovarian Carcinoma Cell Line (NIH:OVCAR-3) with Androgen and Estrogen Receptors Cancer Research. ,vol. 43, pp. 5379- 5389 ,(1983)
Alan N. Houghton, Herbert F. Oettgen, Richard J. Cote, Lloyd J. Old, Edward J. Beattie, Donna M. Morrissey, Human monoclonal antibodies to cancer cells ,(1984)
R. C. Bast, V. R. Zurawski, T. L. Klug, H. M. Davis, Characterization of the CA 125 Antigen Associated with Human Epithelial Ovarian Carcinomas Cancer Research. ,vol. 46, pp. 6143- 6148 ,(1986)
Paula S. Shaw, May-Kin Ho, Howard Sands, Gary L. Davis, Beverly A. Brown, Paul Simon, Jo Saltzgaber-Muller, Gordon P. Moore, Barry A. Stone, Tumor-specific Genetically Engineered Murine/Human Chimeric Monoclonal Antibody Cancer Research. ,vol. 47, pp. 3577- 3583 ,(1987)
H. Sakahara, M. Kunimatsu, Y. Kawamura, K. Endo, H. Ohta, M. Koizumi, T. Nakamura, T. Nakashima, H. Tanaka, Y. Kotoura, Localization of human osteogenic sarcoma xenografts in nude mice by a monoclonal antibody labeled with radioiodine and indium-111 The Journal of Nuclear Medicine. ,vol. 28, pp. 342- 348 ,(1987)
Jorge A Carrasquillo, James L Mulshine, Paul A Bunn, James C Reynolds, Kenneth A Foon, Robert W Schroff, Patricia Perentesis, Ronald G Steis, Andrew M Keenan, Mitchell Horowitz, Steven M Larson, Indium-111 T101 monoclonal antibody is superior to iodine-131 T101 in imaging of cutaneous T-cell lymphoma. The Journal of Nuclear Medicine. ,vol. 28, pp. 281- 287 ,(1987)
Yasutaka Kawamura, Yushi Nishimura, Keigo Endo, Mitsuru Koizumi, Junji Konishi, Yuji Watanabe, Masatoshi Yokoyama, Takeshi Watanabe, Ryuzo Ueda, Tsuneo Saga, In Vitro and In Vivo Properties of Human/Mouse Chimeric Monoclonal Antibody Specific for Common Acute Lymphocytic Leukemia Antigen The Journal of Nuclear Medicine. ,vol. 31, pp. 1077- 1083 ,(1990)
Stephen J. Mather, David Ellison, Reduction-mediated technetium-99m labeling of monoclonal antibodies. The Journal of Nuclear Medicine. ,vol. 31, pp. 692- 697 ,(1990)
K Torizuka, H Sakahara, Y Ohmomo, T Furukawa, K Endo, H Ohta, M Koizumi, T Nakashima, A Yokoyama, K Okada, Effect of DTPA conjugation on the antigen binding activity and biodistribution of monoclonal antibodies against alpha-fetoprotein. The Journal of Nuclear Medicine. ,vol. 26, pp. 750- 755 ,(1985)