Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L.

摘要: Background: Dracocephalum peregrinum L., a traditional Kazakh medicine, has good expectorant, anti-cough, and to some degree, anti-asthmatic effects. Diosmetin (3′,5,7-trihydroxy-4′-methoxyflavone), natural flavonoid found in Chinese herbs, is the main D. L. been used various medicinal products because of its anticancer, antimicrobial, antioxidant, estrogenic, anti-inflammatory The present study aimed investigate effects diosmetin on proliferation, invasion, migration glioma cells, as well possible underlying mechanisms. Methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), scratch wound, Transwell assays were demonstrate glioma. Protein levels Bcl-2, Bax, cleaved caspase-3, transforming growth factor-β (TGF-β), E-cadherin, phosphorylated unphosphorylated smad2 smad3 determined by Western blots. U251 cell development progression measured vivo mouse model. Results: inhibited migration, invasion vitro, TGF-β signaling pathway, Bcl-2 expression. In contrast, there was significant increase caspase-3 Furthermore, it effectively reduced tumorigenicity cells promoted apoptosis vivo. Conclusion: results this suggest that suppresses vitro vivo, possibly activating E-cadherin expression inhibiting pathway.