Highly Red Light-Emitting Erbium- and Lutetium-Doped Core-Shell Upconverting Nanoparticles Surface-Modified with PEG-Folic Acid/TCPP for Suppressing Cervical Cancer HeLa Cells

摘要: Photodynamic therapy (PDT) combined with upconverting nanoparticles (UCNPs) are viewed together as an effective method of ablating tumors. After absorbing highly tissue-penetrating near-infrared (NIR) light, UCNPs emit a shorter wavelength light (~660 nm) suitable for PDT. In this study, we designed and prepared red fluorescence-emitting silica-coated core-shell modified polyethylene glycol (PEG5k)-folic acid tetrakis(4-carboxyphenyl)porphyrin (TCPP) (UCNPs@SiO2-NH2@FA/PEG/TCPP) efficient photodynamic agent killing tumor cells. The consisted two simple lanthanides, erbium lutetium, the core shell, respectively. unique combination enabled to without green light. TCPP, folic acid, PEG were conjugated outer silica layer photosensitizing agent, ligand attachment, dispersing stabilizer, ~50 nm diameter -34.5 mV surface potential absorbed 808 emitted ~660 Most notably, these physically well dispersed stable in aqueous phase due attachment able generate singlet oxygen (1O2) high efficacy. HeLa cells treated each UCNP sample (0, 1, 5, 10, 20, 30 μg/mL free TCPP). results showed that UCNPs@SiO2-NH2@FA/PEG/TCPP laser was significantly cytotoxic cells, almost same degree naive TCPP plus 660 based on MTT Live/Dead assays. Furthermore, internalized into three-dimensional spheroids, presumably small size conjunction endocytosis nonspecific uptake. We believe our will serve new platform deep-penetrating agents various treatments.