Organization of a globular protein film on solid surfaces: an X-ray photoelectron spectroscopy study
作者： Muriel Subirade , Albert Lebugle
DOI: 10.1016/0040-6090(93)04014-J
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摘要: Abstract The organization of legumin films deposited on different solid substrates using the Langmuir-Blodgett technique has been studied by X-ray photoelectron spectroscopy (XPS). XPS data provide information identity chemical elements present as well bonding. From decomposed C 1s spectra, types carbon (peptidic, aliphatic etc.) have identified. variation in atomic ratios a function take-off angle shows structural orientation protein which hydrophobic amino acids are at external surface whereas hydrophilic oriented towards substrate. Moreover, attenuation S 2p signal intensity with decreasing due to layer suggests that adsorbed forms continuous overlayer glass substrate weak dependence F an incomplete coverage polytetrafluoroethylene
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