Nitroxide Radical-Containing Redox Nanoparticles Protect Neuroblastoma SH-SY5Y Cells against 6-Hydroxydopamine Toxicity.

摘要: Parkinson’s disease (PD) patients can benefit from antioxidant supplementation, and new efficient antioxidants are needed. The aim of this study was to evaluate the protective effect selected nitroxide-containing redox nanoparticles (NRNPs) in a cellular model PD. Antioxidant properties NRNPs were studied cell-free systems by protection dihydrorhodamine 123 against oxidation 3-morpholino-sydnonimine fluorescein bleaching 2,2-azobis(2-amidinopropane) hydrochloride sodium hypochlorite. Model blood-brain barrier penetration using hCMEC/D3 cells. Human neuroblastoma SH-SY5Y cells, exposed 6-hydroxydopamine (6-OHDA), used as an vitro Cells preexposed or free nitroxides (TEMPO 4-amino-TEMPO) for 2 h treated with 6-OHDA 1 h 24 h. reactive oxygen species (ROS) level estimated dihydroethidine Fluorimetric Mitochondrial Superoxide Activity Assay Kit. Glutathione (GSH) measured ortho-phtalaldehyde, ATP luminometry, changes mitochondrial membrane potential JC-1, mass 10-Nonyl-Acridine Orange. NRNP1, TEMPO, 4-amino-TEMPO (25-150 μM) protected cells 6-OHDA-induced viability loss; much higher NRNP1 than nitroxides. better permeated BBB Exposure decreased GSH after increased it considerably 24 h (apparently compensatory overresponse); prevented increase. decrease intracellular ROS superoxide level. Studied mostly levels. mass; both effects These results suggest that mitochondria main site damage demonstrate