The preparation, germination properties and stability of superdormant spores of Bacillus cereus.

作者: S. Ghosh , P. Setlow

DOI: 10.1111/J.1365-2672.2009.04442.X

关键词:

摘要: Aims:  To determine yields, germination and stability of superdormant Bacillus cereus spores. Methods Results:  Superdormant B. cereus spores were isolated by with high concentrations inosine or l-alanine in 2–5% yield did not germinate either these germinants, but germinated like starting Ca-DPA, dodecylamine, plus concentrated complete medium. Yields from germinations low higher, poorly inosine, relatively normally inosine. also higher when nonheat-activated germinated. stored at 4°C slowly recovered some capacity, recovery was slowed significantly −20°C −80°C. Conclusions:  Factors that influence levels the properties such are similar to those B. megaterium B. subtilis, suggesting there common mechanisms involved superdormancy spores. Significance:  a major concern food industry, because presence precludes decontamination strategies based on triggering spore followed mild killing treatments. Studies may suggest ways eliminate them.

参考文章(26)
Peter Setlow, Eric A. Johnson, Spores and Their Significance Food microbiology: fundamentals and frontiers. pp. 23- 63 ,(2019) , 10.1128/9781555819972.CH2
B Setlow, P Setlow, Role of DNA repair in Bacillus subtilis spore resistance. Journal of Bacteriology. ,vol. 178, pp. 3486- 3495 ,(1996) , 10.1128/JB.178.12.3486-3495.1996
Luc M. Hornstra, Ynte P. de Vries, Marjon H. J. Wells-Bennik, Willem M. de Vos, Tjakko Abee, Characterization of germination receptors of Bacillus cereus ATCC 14579. Applied and Environmental Microbiology. ,vol. 72, pp. 44- 53 ,(2006) , 10.1128/AEM.72.1.44-53.2006
Luc M. Hornstra, Ynte P. de Vries, Willem M. de Vos, Tjakko Abee, Influence of Sporulation Medium Composition on Transcription of ger Operons and the Germination Response of Spores of Bacillus cereus ATCC 14579 Applied and Environmental Microbiology. ,vol. 72, pp. 3746- 3749 ,(2006) , 10.1128/AEM.72.5.3746-3749.2006
Madan Paidhungat, Peter Setlow, Localization of a germinant receptor protein (GerBA) to the inner membrane of Bacillus subtilis spores. Journal of Bacteriology. ,vol. 183, pp. 3982- 3990 ,(2001) , 10.1128/JB.183.13.3982-3990.2001
Sonali Ghosh, Peter Setlow, Isolation and Characterization of Superdormant Spores of Bacillus Species Journal of Bacteriology. ,vol. 191, pp. 1787- 1797 ,(2009) , 10.1128/JB.01668-08
Christopher Steichen, Ping Chen, John F. Kearney, Charles L. Turnbough,, Identification of the Immunodominant Protein and Other Proteins of the Bacillus anthracis Exosporium Journal of Bacteriology. ,vol. 185, pp. 1903- 1910 ,(2003) , 10.1128/JB.185.6.1903-1910.2003