作者: José A. Siles , Caio T. C. C. Rachid , Inmaculada Sampedro , Inmaculada García-Romera , James M. Tiedje
DOI: 10.1371/JOURNAL.PONE.0103035
关键词:
摘要: The Mediterranean basin has been identified as a biodiversity hotspot, about whose soil microbial diversity little is known. Intensive land use and aggressive management practices are degrading the soil, with consequent loss of fertility. organic amendments such dry olive residue (DOR), waste produced by two-phase olive-oil extraction system, proposed an effective way to improve properties. However, before its application DOR needs pre-treatment, ligninolytic fungal transformation, e.g. Coriolopsis floccosa. present study aimed describe bacterial in assess impact raw (DOR) C. floccosa-transformed (CORDOR) on function phylogeny communities after 0, 30 60 days. Pyrosequencing 16S rRNA gene demonstrated that was dominated phyla Proteobacteria, Acidobacteria, Actinobacteria, while 28S-rRNA data revealed Ascomycota Basidiomycota accounted for majority community. A Biolog EcoPlate experiment showed CORDOR decreased functional altered structures. These changes functionality occurred parallel those phylogenetic community Some groups increased others depending relative abundance beneficial toxic substances incorporated each amendment. In general, observed be more disruptive than CORDOR.