Nosocomial infection caused by vancomycin-susceptible multidrug-resistant Enterococcus faecalis over a long period in a university hospital in Japan

摘要: Compared with other developed countries, vancomycin-resistant enterococci (VRE) are not widespread in clinical environments Japan. There have been no VRE outbreaks and only a few strains sporadically isolated our university hospital Gunma, To examine the drug susceptibility of Enterococcus faecalis nosocomial infection caused by non-VRE strains, retrospective surveillance was conducted hospital. Molecular epidemiological analyses were performed on 1711 E. isolates collected over 6-year period [1998-2003]. Of these isolates, 1241 (72.5%) antibiotic resistant 881 (51.5%) to two or more drugs. The incidence multidrug (MDR-Ef) intensive care unit increased after enlargement restructuring major group MDR-Ef consisted 209 (12.2%) five combination tetracycline/erythromycin/kanamycin/streptomycin/gentamicin. Pulsed-field gel electrophoresis analysis showed that infections long (more than 3 years). Multilocus sequence typing mainly grouped into ST16 (CC58) ST64 (CC8). Mating experiments suggested resistances encoded conjugative transposons (integrative elements), one tetracycline-resistance erythromycin/kanamycin/streptomycin/gentamicin-resistance. knowledge, this is first report vancomycin-susceptible