Small-interfering RNA-mediated identification and regulation of the ternary SNARE complex mediating RBL-2H3 mast cell degranulation.

摘要: Dysregulation of mast cell function contributes to allergic and autoimmune disease that affects more than 70 million persons in the United States alone. Identifying novel targets mediate or progression is required for development innovative therapeutics treatment allergy/asthma disease. RNA interference technologies offer hope both as basic research tools target identification potential, novel, specific therapeutics. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are a family evolutionarily conserved proteins have been postulated transport fusion inflammatory mediator-laden vesicles membrane cells leading their subsequent exocytosis. The functional role(s) SNARE member complexes degranulation has not fully elucidated. Here, we characterize importance FceRI receptor-mediated RBL-2H3 utilizing interference. We demonstrate ternary synaptosomal-associated protein-23, Syntaxin 4 vesicle-associated protein-7 (VAMP-7) VAMP-8 directly involved degranulation. Additionally, evaluate siRNAs directed against these molecules potential therapeutic agents intervention. These studies identified serve siRNA therapies treat