Analysis and Chemical Modification of Bacterial Porins

摘要: The cell envelope of gram-negative bacteria such as Escherichia coli, Salmonella typhimurium, and Pseudomonas aeruginosa consists three different layers, the outer membrane, peptidoglycan (murein) layer, inner membrane (Nikaido, 1979a; Beveridge, 1981). acts a real diffusion barrier contains, in addition to respiration chain H+ -ATPase, large number transport systems for substrates. or murein layer repeating N-acetylglucosaminyl-N-acetylmuramyl dimers, which form macromolecule surrounding (Beveridge, This protects cells from lysis. is molecular filter with defined exclusion limits hydrophilic substrates (Nikaido Nakae, 1979). In enteric bacteria, also hydrophobic compounds detergents 1979b). active sieving properties substances are major class proteins called matrix (Rosenbusch, 1974) porins (Nakae, 1976).