EFFECTS OF MICROGRAVITY AND OXIDATIVE STRESS ON HUMAN T LYMPHOCYTES: DIFFERENT APPROACHES TO STUDY THEIR PROTEIN EXPRESSION AND POST-TRANSLATIONAL MODIFICATIONS

摘要: The aim of this research project was to study the protein expression and post-translational modifications human T cells exposed microgravity oxidative stress. We analysed components IL2/IL-2R signalling system cytoskeletal known be negatively affected by microgravity. Little information is available regarding changes in phosphorylation their functional consequences subjected This data could a valuable indicator microgravity's impact on cellular metabolism. Clinical problems related disturbed immune cell functions astronauts during long-term space flights anticipated prevented. Many pathologies seem directly or indirectly linked phenomena, which influence changes. Such have been associated with molecular switch pathways that regulate proliferation carcinogenesis. In thesis, response extreme conditions evaluated better clarify chemical complexity lymphocytic proteome. Particularly, I focused Spleen Tyrosine Kinase family components, Syk Zap-70, already literature for behaviour pathological states possible role treatment some forms leukemia lymphoma. Western blot analysis total proteins treated different ways showed strong phosphorylative consistent kinase.