Bioactive Assessment and Bacteria Test for the Varied Degrees of Ultra-violet Radiation onto the Collagen-immobilized Polypropylene Non-Woven Fabric

摘要: Exposure to ultra-violet (UV)-C radiation is a frequently used method prevent bacteria from invasion of blood-contact biomedical products. Potential damage induced by UV collagen concern due the decay bioactivity, considerably correlated with structural alterations. Current investigation indicates collagen-immobilized non-woven polypropylene (PP) fabrics sample temperature ca. 4 °C; samples are then exposed UV-254 nm for different time intervals. Using Fourier-Transformed Infrared Attenuated Total Reflection (FTIR-ATR) and XPS (X-ray Photoelectron Spectroscopy), we examine chemical structures treatments. Blood-clotting effects on modified assessed activated partial thromboplastin time, thrombin fibrinogen concentration tests. By means cell counter Scanning Electron Microscopy count red blood cells platelets adhesion in porous matrix. Applying standard plate tests, E. coli, Bacillus stearothermophilus, Staph. aureus, P. aeruginosa, Candida albieans applied. For human plasma incubated various intervals radiation, decreases plasma, while adhesions increase before radiation. The required thrombination shows significant change exposure less than 20 hrs (α = 0.05). Surface analyses indicate that decrease R-COOH (derivated grafted-pAAc or decarboxylation collagen), amides degradation (broken–NH), phenylalanine scission (terminated −OH, tyrosine formation) may gradually increasing measurements C 1s core levels at 288.1 eV (O=C-NH) 289.3 (O=C-O) illustrate intensity after 44 hrs. It clear has potential impact moderate bioactivities therefore act as vital factor accelerate bio-degradation. Bacteria test also supports around no clone formation found immobilized collagen. However, relation between eventual bioactivity capability proliferation should be measured.