rfb Mutations in Vibrio cholerae Do Not Affect Surface Production of Toxin-Coregulated Pili but Still Inhibit Intestinal Colonization

摘要: The toxin-coregulated pilus (TCP) of Vibrio cholerae is essential for colonization. It was recently reported that rfb mutations in V. 569B cause the translocation arrest structural subunit TCP, raising possibility colonization defects lipopolysaccharide mutants are due to effects on TCP biogenesis. However, an rfbB gene disruption either O395 or has no apparent effect surface production as assessed by immunoelectron microscopy and CTX phage transduction, rfbD::Tn5lac mutant also shows defect expression. We conclude associated with unrelated assembly.