Method of detecting a polynucleotide sequence and labelled polynucleotides useful therein

摘要: A method of detecting nucleic acid sequences and enzyme-labelled reagents for use therein. According to the invention sample polynucleotide is first hybridised with a probe comprising seuqence complementary that which be detected, having tail, preferably single stranded poly(dA) or poly(dT) tail. Following attachment sample, an enzyme-containing marker, attached probe, marker single-stranded portion, base groups are one more enzymatically active groups, tail can by annealing hybridisation. Preferably has on whereby second hybridisation step. Presence sequence detected confirmed testing labelled any enzyme related activity.