The crystal and molecular structure of the third domain of silver pheasant ovomucoid (OMSVP3).

摘要: OMSVP3 and OMTKY3 (third domains of silver pheasant turkey ovomucoid inhibitor) are Kazal-type serine proteinase inhibitors. They have been isomorphously crystallized in the monoclinic space group C2 with cell dimensions a= 4.429 nm, b= 2.115 c= 4.405 β= 107°. The asymmetric unit contains one molecule corresponding to an extremely low volume per molecular mass 0.0017 nm3/Da. Data collection was only possible for crystals. Orientation position molecules cells were determined using Patterson search methods known structure third domain Japanese quail (OMJPQ3) [Papamokos, E., Weber, Bode, W., Huber, R., Empie, M. Kato, I. Laskowski, M., Jr (1982) J. Mol. Biol. 158, 515–537]. has refined by restrained crystallographic refinement yielding a final R value 0.199 data 0.15 nm resolution. Conformation hydrogen-bonding pattern OMJPQ3 very similar. Large deviations occur at NH2 terminus owing different crystal packing, C central helix, representing intrinsic property resulting from amino acid substitutions far away this site. deviation complexed Streptomyces griseus protease B is small [Fujinaga, Read, R. J., Sielecki, A., Ardelt, James, N. G. (1982)Proc. Natl Acad. Sci. USA, 79, 4868–4872].