[13] Prenylation-dependent targeting of g-protein-coupled receptor kinases

摘要: Publisher Summary This chapter discusses the translocation–phosphorylation assay used to study cofactors required for membrane or receptor targeting of G-Protein-coupled kinases (GRKs). The family GRKs comprises serine/threonine involved in phosphorylation activated liganded G-protein-coupled receptors. Receptor is a component cellular desensitization, state characterized by loss responsiveness under persistent stimulation also referred as tachyphylaxis, tolerance, adaptation, quenching. rhodopsin kinase (RK) and β -adrenergic 1 ( ARK1), are soluble enzymes that observed redistribute from cytosol membranes cells on activation. measures rhodopsin-mediated G βγ -mediated translocation rod outer segment (ROS) membranes. Detection activity supernatant versus fractions accomplished measuring 32 P incorporation into bleached rhodopsin. procedure outlined useful determining whether translocation-like mechanisms exist newly discovered identifying other components targeting.