Bioconversion of wastewater-derived duckweed to lactic acid through fed-batch fermentation at high-biomass loading

摘要: After being collected from the wastewater treatment system, duckweed biomass which is rich in starch and protein has potential to be converted valuable chemicals. This study aimed establish a fermentation process for bioconversion of duckweed derived lactic acid (LA) by Lactobacillus casei. The casei CICC 23184 showed best LA production ability duckweed-based medium among five strains. Simultaneous saccharification (SSF) separated hydrolysis (SHF) were conducted, SSF had better performance even with lower enzyme dosage. No extra nitrogen source needed supplying into media. In batch fermentation, loading of 220 g kg−1 was optimal concentration 72.51 ± 2.81 g kg−1 yield 0.35 ± 0.01 g g−1dry biomass. highest (100.36 ± 3.31 g kg−1), (0.38 ± 0.01 g g−1dry biomass), productivity (2.09 ± 0.07 g kg−1 h−1) obtained through fed-batch at final 260 g kg−1. demonstrated that it feasible utilize as feedstock produce high-biomass successfully established. provides novel strategy sustainable recycling treatment.