APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria.
作者： Roderick M. Dirkzwager , Andrew B. Kinghorn , Jack S. Richards , Julian A. Tanner
DOI: 10.1039/C5CC00438A
关键词:
摘要: We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit detection = 4.9 ng mL−1) and could reliably diagnose in clinical blood samples.
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A.H. Moody, P.L. Chiodini, Non-microscopic method for malaria diagnosis using OptiMAL IT, a second-generation dipstick for malaria pLDH antigen detection. British Journal of Biomedical Science. ,vol. 59, pp. 228- 231 ,(2002) , 10.1080/09674845.2002.11783665
J. MÜLLER, M. FRIEDRICH, T. BECHER, J. BRAUNSTEIN, T. KUPPER, P. BERDEL, S. GRAVIUS, F. ROHRBACH, J. OLDENBURG, G. MAYER, B. PÖTZSCH, Monitoring of plasma levels of activated protein C using a clinically applicable oligonucleotide-based enzyme capture assay Journal of Thrombosis and Haemostasis. ,vol. 10, pp. 390- 398 ,(2012) , 10.1111/J.1538-7836.2012.04623.X
Michael T. Makler, David J. Hinrichs, Measurement of the lactate dehydrogenase activity of Plasmodium falciparum as an assessment of parasitemia. American Journal of Tropical Medicine and Hygiene. ,vol. 48, pp. 205- 210 ,(1993) , 10.4269/AJTMH.1993.48.205
Lin Cheng, Qiang Zhao, Aptamer-capture based assays for human neutrophil elastase. Talanta. ,vol. 106, pp. 315- 320 ,(2013) , 10.1016/J.TALANTA.2012.11.016
Michael Famulok, Günter Mayer, Aptamer Modules as Sensors and Detectors Accounts of Chemical Research. ,vol. 44, pp. 1349- 1358 ,(2011) , 10.1021/AR2000293
Elena Garcia, Jared R. Kirkham, Anson V. Hatch, Kenneth R. Hawkins, Paul Yager, Controlled microfluidic reconstitution of functional protein from an anhydrous storage depot Lab on a Chip. ,vol. 4, pp. 78- 82 ,(2004) , 10.1039/B308914B
Chih-Hung Wang, Kang-Yi Lien, Jiunn-Jong Wu, Gwo-Bin Lee, A magnetic bead-based assay for the rapid detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system with integrated loop-mediated isothermal amplification Lab on a Chip. ,vol. 11, pp. 1521- 1531 ,(2011) , 10.1039/C0LC00430H
Kasia Stepniewska, Elizabeth Ashley, Sue J Lee, Nicholas Anstey, Karen I Barnes, Tran Quang Binh, Umberto D'Alessandro, Nicholas PJ Day, Peter J De Vries, Grant Dorsey, Jean-Paul Guthmann, Mayfong Mayxay, Paul N Newton, Piero Olliaro, Lyda Osorio, Ric N Price, Mark Rowland, Frank Smithuis, Walter RJ Taylor, François Nosten, Nicholas J White, None, In vivo parasitological measures of artemisinin susceptibility. The Journal of Infectious Diseases. ,vol. 201, pp. 570- 579 ,(2010) , 10.1086/650301
Clare Sansom, Overprescribing of antimalarials Lancet Infectious Diseases. ,vol. 9, pp. 596- ,(2009) , 10.1016/S1473-3099(09)70247-6
David H. J. Bunka, Peter G. Stockley, Aptamers come of age – at last Nature Reviews Microbiology. ,vol. 4, pp. 588- 596 ,(2006) , 10.1038/NRMICRO1458