Mass Spectrometry and NMR Spectroscopy–Based Quantitative Metabolomics

摘要: Abstract Quantitative metabolomics, in which known metabolites biological mixtures are quantitatively analyzed before statistical analysis, is gaining increased importance the rapidly growing field of metabolomics. A major benefit quantitative metabolomics approach compared to global chemometric analysis spectral data that it can reduce potential errors arising from numerous factors including background distortions, strong solvent signals, and peak misalignments. promises reliable insights into systems biology through accurate metabolite concentration measurements. The two most commonly used analytical methods mass spectrometry (MS) nuclear magnetic resonance (NMR) spectroscopy. Numerous approaches within these provide complementary as well supplementary information on concentrations metabolites, their ranges, changes complex mixtures. Current technological advancements MS NMR combined with new experimental computational enable routine hundreds metabolites. This chapter focuses recent developments methods, emphasizing targeted for