Transneuronal labeling of CNS neuropeptide and monoamine neurons after pseudorabies virus injections into the stellate ganglion

摘要: Abstract The viral transneuronal labeling method was used in combination with immunohistochemical procedures to identify CNS neuropeptide and monoamine neurons that innervate the sympathetic preganglionic neuron (SPNs) which project stellate ganglion—the principal source of supply heart. Transneuronal found at three levels: spinal cord, brainstem, hypothalamus. In thoracic apart from pseudorabies virus (PRV)-labeled SPNs, PRV-labeled were localized laminae I/II, IV, V, VII, X as well lateral nucleus funiculus. C1–C4 segments, labeled funiculus V VII gray matter. cells identified lamina dorsolateral lumbar cord. Three medullary areas consistently labeled: rostral ventromedial medulla (RVMM), ventrolateral (RVLM), caudal raphe nuclei. greatest concentration RVMM. This projection arose adrenergic, serotonergic (5-HT), thyrotropin releasing hormone (TRH), substance P, somatostatin, enkephalin, vasoactive intestinal peptide (VIP) immunoreactive neurons. RVLM originated mainly C1 adrenergic neurons, some contained Y (NPY). C3 adrenergic-NPY lying near floor 4th ventricle also labeled. Enkephalin-, somatostatin- VIP-immunoreactive contributed this projection. 5-HT nuclei (raphe pallidus, obscurus, magnus) labeled; these P or TRH-immunoreactivity an occasional staining for all putative neurotransmitters. pons, catecholamine A5 cell group, subcoeruleus Kolliker-Fuse midbrain relatively few infected cells, but present Edinger-Westphal precommissural Forebrain concentrated paraventricular hypothalamic (PVN) lesser amounts area (LHA) perifornical region. PVN, oxytocin-immunoreactive accounted chemically-defined while corticotrophin factor (CRF), vasopressin-, angiotensin II-immunoreactive provided successively inputs. LHA, summary, study provides first detailed map chemically-coded involved control cardiosympathetic outflow.