MicroRNAs as Engineering Targets: Pathway Manipulation to Impact Bioprocess Phenotypes

摘要: Chinese hamster ovary (CHO) cells are the primary mammalian culture system used for recombinant protein production; therefore there continuous research and development efforts to improve cell production capabilities by both genetic modification process optimization strategies. The modifications increase specific growth rate, reduce apoptosis nutrients utilization. Since altering expression of a single gene or even pathway may not be sufficient produce desirable phenotypes, regulation global better approach engineering in CHO cells. miRNA(s) were found regulators with ability simultaneously alter multiple cellular pathways such as growth, apoptosis, stress resistance, metabolism secretion. Therefore, miRNA profiles facilitate design high-producing Recent advances transfection techniques allow insertion mimics inhibitors into at stages bioprocess. Unlike traditional approaches, manipulation does burden translational machinery therefore, metabolic resources allocated production. In this chapter we highlight industrially-relevant pathways, report on involvement their regulation, discuss how these miRNAs can performance industrial applications propose candidates engineering.