MicroRNA-203 Regulates Melanosome Transport and Tyrosinase Expression in Melanoma Cells by Targeting Kinesin Superfamily Protein 5b

摘要: MicroRNA (miR)-203 is known to be downregulated and act as an anti-oncomir in melanoma cells. At present, we found that exogenous miR-203 increased pigmentation protein expression levels of the antigen recognized by T cells (Melan-As/MART1s) and/or tyrosinase (TYR) human tested. Inversely, treatment with inhibitor level TYR. The target gene involved mechanism was kinesin superfamily 5b (kif5b), which revealed silencing using short interfering RNA luciferase activity assay. Furthermore, immunocytochemistry showed obvious accumulation melanosomes around nuclei Mewo transfected or siR-kif5b. Importantly, inhibitor, but not miR-203, exhibited effects on epidermal melanocytes isolated from lightly pigmented adult skin similar those In addition, data indicated also negatively regulated cAMP response element–binding 1 (CREB1)/microphthalmia-associated transcription factor (MITF)/Rab27a pathway, one main pathways active conclusion, our anti-oncogenic had a pivotal role through reducing melanosome transport promoting melanogenesis targeting kif5b negative regulation CREB1/MITF/Rab27a pathway.