Active Oxygen Species Generated from Photoexcited Fullerene (C60) as Potential Medicines: O2-• versus 1O2

摘要: To characterize fullerenes (C60 and C70) as photosensitizers in biological systems, the generation of active oxygen species, through energy transfer (singlet 1O2) electron (reduced radicals such superoxide anion radical O2-• hydroxyl •OH), was studied by a combination methods, including biochemical (DNA-cleavage assay presence various scavengers species), physicochemical (EPR trapping near-infrared spectrometry), chemical methods (nitro blue tetrazolium (NBT) method). Whereas 1O2 generated effectively photoexcited C60 nonpolar solvents benzene benzonitrile, we found that •OH were produced instead polar water, especially physiological concentration reductants NADH. The above results, together with those DNA cleavage specific indicate active...