Ts6 and Ts2 from Tityus serrulatus venom induce inflammation by mechanisms dependent on lipid mediators and cytokine production.

摘要: Abstract Inflammatory mediators are thought to be involved in the systemic and local immune response induced by Tityus serrulatus scorpion envenomation. New functional aspects of lipid have recently been described. Here, we examine unreported role cell recruitment peritoneal cavity after an injection with Ts2 or Ts6 toxins isolated from T. venom. In this report, demonstrate that following a single intraperitoneal (i.p.) (250 μg/kg) mice, there was induction leukocytosis predominance neutrophils observed at 4, 24, 48 96 h. Moreover, total protein, leukotriene (LT)B4, prostaglandin (PG)E2 pro-inflammatory cytokine levels were increased. We also increase regulatory cytokines, including interleukin (IL)-10, injection. Finally, 5-lipoxygenase (LO) deficient mice wild type (WT) 129sv pre-treated LTs PGs inhibitors (MK-886 celecoxib, respectively) reduction influx leukocytes occurs comparison WT. The these cells demonstrated phenotype characteristic neutrophils, macrophages, CD4 CD8 lymphocytes expressing GR1+, F4/80+, CD3+/CD4+ CD3+/CD8+, respectively. conclusion, our data induce inflammation mechanisms dependent on production. may play whereas exhibits activity exclusively.