Cleavage of phycocyanobilin from C-phycocyanin. Separation and mass spectral identification of the products.
作者： R C Pierce , R J Beuhler , H W Siegelman , L Friedman
DOI: 10.1016/S0021-9258(17)33602-5
关键词:
摘要: The chromophore of C-phycocyanin, phycocyanobilin, was cleaved from the protein with methanol, concentrated hydrochloric acid, or subtilisin BPN'. pigments obtained were converted to their dimethyl esters and purified by preparative high pressure liquid chromatography examined for purity analytical on silica gel. They characterized proton transfer electron impact mass spectroscopy. principal product three cleavage procedures phycocyanobilin. Methanol acid adducts phycocyanobilin methanol cleavages, respectively. adduct formation can occur subsequent requires catalysis. No observed mesobiliverdin under similar conditions. These results spectral data support conclusion that takes place at exocyclic olefin linkage ring A in ease co-valent strongly suggests ethylidene side chain is an important binding site polypeptide chain.
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