Prevalence of Human Papillomavirus Types in Invasive Vulvar Cancers and Vulvar Intraepithelial Neoplasia 3 in the United States Before Vaccine Introduction

摘要: OBJECTIVE The study aimed to determine the baseline prevalence of human papillomavirus (HPV) types in invasive vulvar cancer (IVC) and intraepithelial neoplasia 3 (VIN 3) cases using data from 7 US registries. MATERIALS AND METHODS Registries identified eligible diagnosed 1994 2005 requested pathology laboratories prepare 1 representative block for HPV testing on those selected. Hematoxylin-eosin-stained sections preceding following used extraction were reviewed confirm representation. Human was detected L1 consensus polymerase chain reaction (PCR) with PGMY9/11 primers type-specific hybridization, retesting samples negative inadequate results SPF10 primers. For IVC, confirmatory hematoxylin-eosin slides re-evaluated histological type. Descriptive analyses performed examine distributions by histology other factors. RESULTS 121/176 (68.8%) IVC 66/68 (97.1%) VIN (p < .0001). Patients differed median age (70 vs 55 y, p = .003). 16 present 48.6% 80.9% cases; high-risk 19.2% 13.2% cases. Prevalence squamous cell carcinoma subtype .0001) as follows: keratinizing, 49.1% (n 55); nonkeratinizing, 85.7% 14), basaloid, 92.3% warty 78.2% 55), mixed warty/basaloid, 100% 7). CONCLUSIONS Nearly all two thirds positive HPV. ranged across subtypes. Given high cases, prophylactic vaccines have potential decrease incidence neoplasia.