Hypoxia and the Renin-Angiotensin System in Atherosclerosis

作者: Cecilia Thalén Johansson

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摘要: 3 Background 6 Aim 11 Material and methods 12 Histological examination of atherosclerotic plaques – comparative analysis plaque morphology serum markers inflammation between patients receiving ARB treatment controls Study population design Atherosclerotic 14 Serum samples 15 In vitro the effects hypoxia on expression AT1 in cell culture Cell smooth muscle cells 16 monocyte derived macrophages Real time reverse transcriptase polymerase chain reaction (Real Time RT-PCR) Enzyme-linked immunosorbent assay (ELISA) 17 Western Immunoblotting 18 Statistics 19 Results 20 Expression co localizes with CD68 positive HIF-1α human carotid Significant correlation as well 23 Similar levels treated 24 SMC cell-culture experiments proved inconclusive to mRNA 28 was reduced SMCs exposed 29 No change secretion cytokines, ACE or angiotensin II from Candesartan compared 30 Macrophage exposure hypoxia, protein verified 31 Discussion 32

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