Thermodynamic Cooperativity and Kinetic Proofreading in DNA Damage Recognition and Repair

摘要: In humans UV-induced cyclobutane thymine dimers are excised by the joint action of six repair factors, RPA, XPA, XPC, TFIIH, XPG, and XPF.ERCC1. Yet, in vitro assays show that none these factors is capable detectably discriminating dimer-containing DNA from undamaged DNA. We how two elementary principles macromolecular recognition, (1). cooperativity (2). kinetic proofreading, utilized to confer specificity system where exists at individual factor level enable human cells excise with a physiologically relevant biologically acceptable rate.