Cloning and expression in Escherichia coli of a xylanase gene from Bacteroides ruminicola 23.
作者： T R Whitehead , R B Hespell
DOI: 10.1128/AEM.55.4.893-896.1989
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摘要: A gene coding for xylanase activity in the ruminal bacterial strain 23, type of Bacteroides ruminicola, was cloned into Escherichia coli JM83 by using plasmid pUC18. AB. ruminicola 23 genomic library prepared E. BamHI-digested DNA, and transformants were screened on basis clearing areas around colonies grown Remazol brilliant blue R-xylan plates. Six clones identified as being positive, all six contained same 5.7-kilobase insert. The reduced to a 2.7-kilobase DNA fragment. Xylanase produced clone found be greater than that original B. strain. Southern hybridization analysis from related strains, D31d H15a, demonstrated one hybridizing band each DNA. Images
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