Cytokinesis in Trypanosoma brucei differs between bloodstream and tsetse trypomastigote forms: implications for microtubule‐based morphogenesis and mutant analysis

摘要: Trypanosomes use a microtubule-focused mechanism for cell morphogenesis and cytokinesis. We used scanning electron video microscopy of living cells to provide the first detailed description cytokinesis in early-branching eukaryote Trypanosoma brucei. outline four distinct stages show that an asymmetric division fold bisects two daughter cells, with cytoplasmic bridge-like structure connecting daughters immediately prior abscission. Using detection tyrosinated α-tubulin as marker new or growing microtubules expression XMAP215, plus end binding protein, microtubule ends we demonstrate spatial asymmetry underlying cytoskeleton throughout cycle. This leads inheritance different cytoskeletal patterns demonstrates major role achieving RNA interference techniques have led large set mutants, often variations phenotype between procyclic bloodstream life cycle forms. Here, morphogenetic differences these forms this parasite during flagellum growth These discoveries are important tools explain form RNAi phenotypes involving organelle mis-positioning defects.