Hollow manganese phosphate nanoparticles as smart multifunctional probes for cancer cell targeted magnetic resonance imaging and drug delivery

摘要: Multifunctional probes for simultaneous magnetic resonance imaging (MRI) and drug delivery have attracted considerable interest due to their promising potential applications in the early-stage diagnosis therapy of diseases. In this study, hollow manganese phosphate nanoparticles (HMP NPs) with an average diameter 18 nm were synthesized aminated through silanization, which enabled covalent conjugation biocompatible poly(ethylene glycol) (PEG) on surfaces. The anti-tumor doxorubicin (DOX) could be loaded into cavities. Under physiological conditions (pH 7.4), NPs showed low MRI T 1 contrast (r = 1.19 L·mmol−1·s−1), whereas high enhancement 5.22 L·mmol−1·s−1) was achieved after dissolving them endosome/lysosome mimetic 5.4). This is fact that easily eroded, resulted release Mn2+ at pH. To use interesting phenomenon targeted DOX delivery, we conjugated tumor-targeting ligand folic acid (FA) HMP investigated capacity cytotoxicity cell lines expressing different amount folate receptor (FR). KB cells more significant cellular uptake than HeLa A549 cells, as confirmed by confocal laser scanning microscopy (CLSM), flow cytometry 1-weighted MRI. Furthermore, drug-loaded exhibited greater cells. Our results suggest functionalized can act effective multifunctional probe selective MRI, well giving efficient delivery. Open image new window