Apocynin decreases AGEs-induced stimulation of NF-κB protein expression in vascular smooth muscle cells from GK rats.

摘要: AbstractContext: Elevated oxidative stress plays a key role in diabetes-associated vascular disease. Excessive production of reactive oxygen species via advanced glycation end products (AGEs) activates peroxisome proliferator-activated receptor gamma (PPARγ) and the transcription factor nuclear factor-kB (NF-κB) aortic smooth muscle cells (VSMCs). Apocynin, drug with an antioxidant effect, has also been proposed as therapeutic agent for atherosclerotic disease.Objectives: This work investigates effects apocynin on PPARγ NF-κB protein expression evoked by AGEs cultured VSMCs from Goto–Kakisaki (GK) rats, non-obese insulin model both resistance type 2 diabetes.Materials methods: VSMCs, isolated aortas GK non-diabetic were cultured. The proteins was evaluated Western blot. blood glucose concentration measured test meter. diabetes rats controlled det...