P124. 3-Polyunsaturated fatty acids may inhibit angiogenesis through suppression of ELR+ CXC chemokines expression and VEGF signaling in pancreatic cancer

摘要: Angiogenesis, the process of new blood vessel formation, is essential for tumor growth and metastasis. Although protective role ω3-PUFAs on cancer progression has been reported, anti-angiogenic mechanisms remain to be further understood. The aim this study investigate in pancreatic cancer. In angiogenesis protein array chip assay, only angiogenic ELR+ CXC chemokines expression, which activate endothelial cell migration neutrophils recruitment, was significantly reduced by DHA treatment SW1990 human cells. expression chemokine confirmed RT-PCR. Chemotactic HUVEC DHA-treated conditioned medium (CM) compared control CM. also inhibited VEGF reporter activity VEGF-induced proliferation were markedly treatment. MMP-2 MMP-9 promoter activities treatment, ultimately attenuated invasiveness cells as revealed matrigel droplet assay. Moreover, NF-κB activity, closely correlated with chemokine, MMPs, When mouse PANC02 implanted into Fat-1 transgenic mice, size volume dramatically mice mice. immunohistochemical analysis tumors apoptosis (TUNEL positive cells) increased microvessel density (CD31 intensity) addition, neovascularization a subcutaneous plug Our finding suggests that can suppress through inhibition signal Thus supplementation may beneficial chemoprevention effect. This work supported basic Science Research Program National Foundation Korea funded Ministry Education, Technology (R13-2007-020-01000-0 2010-0016447), Korea.