Osteoblast migration on poly(α-hydroxy esters)

摘要: We investigated the migration of rat calvaria osteoblast populations on poly(α-hydroxy ester) films for up to 14 days determine effects substrate composition and culture conditions migratory characteristics osteoblasts. Initial included cell colonies formed by seeding a high (84,000 cells/cm2) or low (42,000 density isolated osteoblasts polymer films, bone tissue cultures plating chips directly substrates. High cultured allowed migrate proliferate radially 85:15 poly(DL-lactic-co-glycolic acid) (PLGA) 75:25 PLGA polystyrene controls demonstrated that copolymer ratio in did not affect rate increase surface area (or area) covered growing colony. However, was dependent initial density. with lower resulted area, specifically 4.9 ± 0.3 mm2/day, versus 14.1 0.7 mm2/day seeded higher cells same films. The proliferation differ, whereas arising from than either these colonies. Confocal light microscopy revealed occurred as monolayer individual calcified front. These results strongly rates biodegradable esters). © 1996 John Wiley & Sons, Inc.