The Berberis aristata Ameliorates oxazolone induced contact dermatitis: in-vivo and in silico evidences

摘要: The objective of the present study was to evaluate effect Berberis aristata (BA) against oxazolone induced contact dermatitis in Balb/c mice and molecular docking with TLR-1 TLR-2. Dermatitis by sensitizing topical application 100 μl (2%) BA screened two doses (200 mg/kg 400 mg/kg P.O.). evaluated change ear thickness, anti-inflammatory cytokine (TNF-α, IL-6, IL-1β) oxidative stress on sixth day tissue homogenate. skin all group subjected histological analysis. This work further evidenced berberine crystal structure TLR1-TLR2 heterodimer caused using binding tri-acylated lipopeptide (PDB ID: 2Z7X). In this study, we found that a significant reduction thickness 400 mg/kg) as compared oxazolone(2%) treated mice. level GSH SOD sensitized animals showed an increase levels. A inflammatory cytokines observed indicates activity oxazolone. Histopathological analysis minimal infiltration lymphocytes moderate harm cells layer Docking studies revealed promising interaction withTLR1-TLR2 which can attribute its anti dermatic effect. research shows has dose-dependent attenuated