A TRP Channel Senses Lysosome Neutralization by Pathogens to Trigger Their Expulsion

摘要: Vertebrate cells have evolved elaborate cell-autonomous defense programs to monitor subcellular compartments for infection and evoke counter-responses. These are activated by pathogen-associated pattern molecules various strategies intracellular pathogens employ alter cellular microenvironments. Here, we show that, when uropathogenic E. coli (UPEC) infect bladder epithelial (BECs), they targeted autophagy but avoid degradation because of their capacity neutralize lysosomal pH. This change is detected mucolipin TRP channel 3 (TRPML3), a transient receptor potential cation localized lysosomes. TRPML3 activation then spontaneously initiates lysosome exocytosis, resulting in expulsion exosome-encased bacteria. studies reveal default system homeostasis that has been co-opted the autonomous program clear recalcitrant pathogens.