Both the cytosols and detergent extracts of breast cancer tissues are suited to evaluate the prognostic impact of the urokinase-type plasminogen activator and its inhibitor, plasminogen activator inhibitor type 1.

摘要: The serine protease urokinase-type plasminogen activator (uPA) plays a key role in tumor-associated proteolysis malignant solid tumors. Proteolytic activity of uPA is controlled by its naturally occurring inhibitor type 1. As an initial observation, correlation enzymatic breast cancer cytosols with prognosis was described 1988 (Duffy et al. , Cancer (Phila.), 62: 531–533, 1988). A pronounced prognostic impact uPA, independent classical risk parameters, then first demonstrated detergent-extracted (Triton X-100) tissues applying enzyme-linked immunosorbent assay techniques (Janicke Lancet, 2: 1049, 1989; Fibrinolysis, 4:69–78, 1990; Duffy Res., 50: 6827–6829, 1990). In addition, not only but also 1 were shown to be value Semin. Thromb. Hemostasis, 17: 303–312, 1991; Breast Res. Treat., 24: 195–208, 1993). Subsequently, the and confirmed studies using archived ‘cytosol fractions” (Foekens 52: 6101–6105, 1992; Spryatos J. Natl. Inst., 84: 1266–1272, Grondahl-Hansen 53: 2513–2521, 1993; Sumiyoshi Int. Cancer, 345–348, 1992). direct comparison both methods regard prognosis, however, lacking. We therefore prepared detergent-treated tissue extracts cytosol fractions from same specimens allow methods. In 247 patients investigated, Triton X-100-extracted revealed about twice as much antigen (uPATx: median, 2.32 ng/mg protein) than (uPAcyt: 1.07 protein). contrast, presence X-100 did result increase PAI-1 (PAI-1Tx: 6.34 ng PAI-1/mg compared (PAI-1cyt: 7.15 Good correlations between uPATx uPAcyt ( R = 0.72) PAI-1Tx PAI-1cyt - 0.88) observed. Furthermore, are moderately correlated each other (uPATx versus PAI-1Tx: 0.40; PAI-1cyt: 0.39). power showed best advantage RR 3.22), most subgroups node-negative premenopausal patients, respectively. influenced extraction procedure 3.15). strong parameters (multivariate analysis), simultaneous determination factors recommended yield optimal information, preferentially extracts.