Physico-chemical characterization of the interaction of red fluorescent protein — DsRed with silica layers

摘要: The Discosoma Recombinant Red Fluorescent (DsRed) protein is the latest member of family fluorescent proteins. It holds great promise for applications in biotechnology and cell biology. However, before being used rational engineering, knowledge on underlying mechanisms relating DsRed structural stability adsorption properties solid surfaces highly demanded. physico-chemical analysis performed this study reveals that interaction with SiO2 does not lead to denaturation. secondary structure preserved after dehydration. measured contact angles sessile droplets different concentrations determine as hydrophilic one. photoluminescence emission dehydrated found be slightly red-shifted, peaking at 590 nm.