Evaluation of cytogenetic and DNA damage induced by the antibacterial drug, trimethoprim.
作者： A. Abou-Eisha
DOI: 10.1016/J.TIV.2005.10.008
关键词:
摘要: Trimethoprim, a widely used antimicrobial drug was tested for its effect on the level of nuclear DNA damage in cultured peripheral blood lymphocytes terms chromosome and alterations. The extent cytogenetic damage, expressed as breakage loss, evaluated employing cytokinesis block micronucleus method (CBMN) coupled with fluorescence situ hybridization (FISH) using digoxigenin-labelled alphoid probe specific centromere all human chromosomes. by Comet assay. Cultures were set up samples from two healthy donors. A range concentrations test agent (from 1 to 100 microg/ml) micronuclei (MN) frequency origin MN. For assay doses 0.5 150 microg/ml. From results obtained it appears that this antifolic has significant clastogenic potential, detected dose-dependent increase incidence C-MN significantly greater than control levels at highest (25,100 microg/ml). In addition, also show trimethoprim induces breakage, attaining statistical significance (25, 100, microg/ml), which would confirm genotoxicity.
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