Analysis of Hantaan Virus RNA: Evidence for a New Genus of Bunyaviridae

摘要: Abstract Hantaan virus, the prototype virus of hemorrhagic fever with renal syndrome, was examined for nucleic acid characteristics which would support its previously proposed inclusion in family Bunyaviridae. Nucleocapsid RNA from virions and a control bunyavirus were ribonuclease A (RNase A) sensitivity. Both viruses exhibited similar accessibility within nucleocapsids to digestion by RNase A. Complete both affected high concentrations ribonuclease. Evidence negative strand polarity obtained an vitro transcriptase assay. dependent polymerase activity associated virions. Polymerase required manganese nucleoside triphosphates enhanced magnesium, 2-mercaptoethanol, sodium chloride. Oligonucleotide map analysis large (L), medium (M), small (S) genome segments demonstrated that each species unique respect other different host cell ribosomal RNA. common 3′ terminal sequence three determined be AUCAUCAUCUG . This is those reported four recognized genera Because all data consistent Bunyaviridae, we propose separate genus Bunyaviridae as virus.