Plasma polymerised nanoscale coatings of controlled thickness for efficient solid-phase presentation of growth factors.

摘要: The engineering of biomaterial surfaces and scaffolds for specific biomedical clinical application is growing interest. Certain functionalised can capture deliver bioactive molecules, such as growth factors (GF), enhancing the efficacy systems. With a custom-made plasma polymerisation reactor described here we have developed polymer coatings based on poly(ethyl acrylate) (PEA). This remarkable unfolds fibronectin (FN) upon adsorption to allow GF binding region FN sequester present GFs with high efficiency. We systematically evaluate process conditions their impact polymerised PEA characterise effect power deposition time thickness, wettability chemical composition coatings. demonstrate that functional substrate roughness be maintained after Importantly, show deposited at different all maintain similar or better bioactivity than spin coated references. in assembles into nanonetworks availability integrin regions bone morphogenetic protein-2 (BMP-2). also report mesenchymal stem cell adhesion behaviour, characterised by focal adhesions, differentiation potential BMP-2 surfaces, regardless conditions. potent versatile technology help facilitate use applications.