Single cell transcriptomic analysis of murine lung development on hyperoxia-induced damage.

摘要: During late lung development, alveolar and microvascular development is finalized to enable sufficient gas exchange. Impaired manifests as bronchopulmonary dysplasia (BPD) in preterm infants. Single-cell RNA sequencing (scRNA-seq) allows for assessment of complex cellular dynamics during biological processes, such development. Here, we use MULTI-seq generate scRNA-seq profiles over 66,000 cells from 36 mice normal or impaired secondary hyperoxia with validation some the findings lungs BPD patients. We observe dynamic populations cells, including several rare cell types putative progenitors. Hyperoxia exposure, which mimics phenotype, alters composition all compartments, particularly epithelium, stromal fibroblasts, capillary endothelium macrophage populations. Pathway analysis predicted crosstalk suggest inflammatory signaling main driver hyperoxia-induced changes. Our data provides a single-cell view changes associated health disease.