Identification of the very early transcribed baculovirus gene PE-38.

摘要: We have started to identify early viral RNAs that are transcribed at 1 h after inoculation investigate the mechanism involved in regulation of gene expression Autographa californica nuclear polyhedrosis virus (AcNPV). Cloned DNA fragments were hybridized Northern (RNA) blots polyadenylated RNA isolated from Spodoptera frugiperda cells 1, 2, and 6 postinfection localize very transcripts. Subsequently we prepared a cDNA library analyze clones corresponding major RNAs. identified located upstream immediate-early IE-N extending opposite direction. Because during AcNPV infection transient uninfected cells, conclude found an gene, designated PE-38. The determination nucleotide sequence PE-38 revealed one open reading frame potentially encoding product 38 kDa. Results vitro translation experiments suggest PE-38-specific polypeptide approximately kDa can be expressed. evidence computer analyses predicted amino acid includes two putative DNA-binding motifs, zinc finger, leucine zipper.