Non-nucleic acid probes, probe sets, methods and kits pertaining to the detection of individual human chromosomes X, Y, 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 16, 17, 18 and 20 as 13/21 as a pair

摘要: This invention is related to novel non-nucleic acid probes, probe sets, methods and kits pertaining the detection, identification or quantitation of human chromosomes X, Y, 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 13, 16, 17, 18, 20 and/or 21. The this are particularly well suited for use in multiplex ISH FISH assays wherein each 18 20, as 13/21 a pair, sample cell can be individually detected, identified quantitated same assay. Multiplex possible because two more probes used assay labeled with one independently detectable labels. Preferably, labels fluorophores. In preferred embodiments, comprise linked moieties combination detect, identify quantitate particular probe/target sequence hybrid. methods, kits, sets automated analysis, including slide scanner based system, microscope CCD camera flow cytometer. Furthermore, useful detection identifying chromosome abnormalities such aneuploidy polyploidy karyotypes preimplantation diagnosis, prenatal screening clinical diagnostic applications.