Studies on metabolic role of 5'-Methylthioadenosine in Ochromonas malhamensis and other microorganisms.

摘要: Several compounds containing a thiomethyl group were found to replace vitamin B12 in protozoan, Ochromonas malhamensis. The order of the effectiveness was as follows: 5′-methylthioadenosine > S-adenosylmethionine 5-methylthioribose L-methionine. A similar obtained with respect permeability these into protozoan cells, except for S-adenosylmethionine. 5′-Methylthioadenosine and well l-methionine markedly increased intracellular content l-methionine. level also by them, but lesser degree. established be incorporated metabolic fate cannot distinguished from that high activity nucleosidase detected cell-free extracts protozoan. These results strongly suggest would metabolized via then converted Like B12, may play an important role maintenance C-1 pool