Circular dichroism of fusarium lipoxygenase from Fusarium oxysporum

摘要: Summary In the Soret region, a large negative trough at 427.5 nm of oxidized Fusarium lipoxygenase between positive peaks 400 and 490 was observed. But CD spectrum reduced enzyme considerably changed many complex with amplitudes were From these results, protoheme IX in might be distinguished from that cytochrome b its function. The spectral conversion pH 6.0(α-helix form) 9.0(β far-ultraviolet region observed this entirely reversible. At 12.0, however, band greatly diminished, suggesting great change backbone structure. This considerable conformational 12.0 will explain well fact positional specificity hydroperoxidation depending on also has highest activity due to accessibility substrate IX.