Murine pancreatic ductal adenocarcinoma produced by in vitro transduction of polyoma middle T oncogene into the islets of Langerhans.

摘要: Pancreatic islets isolated from juvenile but not aging adult mice, when infected with a retrovirus carrying polyomavirus middle T oncogene, produced cell lines, mPAC, characteristics both of pancreatic ductal epithelium and neuroendocrine cells the islets. Following three cycles single cloning, mPAC consisted two subtypes, null cell, double-positive that co-expressed cytokeratin, marker epithelium, A2B5, ganglioside expressed in developing islet cells. Two genes, encoding somatostatin polypeptide, were transcribed at low levels most clones, whereas insulin glucagon genes not. Upon inoculation rapidly formed well-differentiated adenocarcinomas cytokeratin markers. The phenotype may result specific dedifferentiation or induced by protein. Alternatively, arise transformation multipotential progenitor present within juxtaposition to This type could therefore represent one targets human cancers duct. Moreover, signal transduction systems modulated T, including src-related kinases, phosphatidylinositol kinase, protein phosphatase 2A, be involved carcinogenesis.