Unravelling the complete genome sequence of Advenella mimigardefordensis strain DPN7T and novel insights in the catabolism of the xenobiotic polythioester precursor 3,3′-dithiodipropionate

摘要: Advenella mimigardefordensis strain DPN7T is a remarkable betaproteobacterium because of its extraordinary ability to use the synthetic disulfide 3,3′-dithiodipropionic acid (DTDP) as sole carbon source and electron donor for aerobic growth. One application DTDP precursor substrate biotechnically synthesized polythioesters (PTEs), which are interesting non-degradable biopolymers applicable plastics materials. Metabolic engineering optimization PTE production requires an understanding conversion. The genome A. was sequenced annotated. circular chromosome found be composed 4 740 516 bp 4112 predicted ORFs, whereas plasmid consisted 23 610 24 ORFs. genes participating in catabolism had been characterized detail previously, but knowing complete sequence with support Tn5 : : mob-induced mutants, putatively involved transporter proteins transcriptional regulator were also identified. Most probably, transported into cell by specific tripartite tricarboxylate transport system then cleaved reductase LpdA, sulfoxygenated 3-mercaptopropionate dioxygenase Mdo, activated CoA ligase SucCD desulfinated acyl-CoA dehydrogenase-like desulfinase AcdA. Regulation this pathway presumably performed xenobiotic response element family. excessive sulfate that inevitably produced secreted cells unique exporter CPA (cation : proton antiporter) superfamily.