Covalent Labeling of Nucleosides with VIII‐ and VI‐Valent Osmium Complexes
作者： Mojmír Trefulka , Veronika Ostatná , Luděk Havran , Miroslav Fojta , Emil Paleček
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摘要: Osmium tetroxide complexes with nitrogen ligands [Os(VIII)L] have been widely applied as probes of the DNA structure and electroactive labels DNA. Here we describe electrochemical behavior Os(VIII)2,2-bipyridine (Os, bipy)-base-labeled nucleosides. We show that label can be introduced also in nucleoside ribose residues using six-valent osmium complex. Cyclic voltammograms sugar-Os(VI)-modified ribosides are similar but not identical to those base-modified ribosides. Our results showing electroactivity sugar modified pave way facile end-labeling RNA.
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